By Rodolfo Paoletti; David Kritchevsky
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Additional resources for Advances in lipid research. Vol. 18, 1981
Preliminary bromination was also shown to be effective with other lipid stains, such as Oil red O a n d Nile Blue sulfate (Adams, 1969). The general enhancement of lipid staining after bromination might be a specific chemical effect of bromine o n lipids; however, it is also possible that it is due to a reduced solubility of lipids in aqueous and other fluids. Crystals of free cholesterol, confirmed with the Schultz and perchloric a c i d - n a p h t h o quinone methods (Adams, 1969), can also be identified by this simple technique.
T o demonstrate apo-B localization Hoff and G a u b a t z (1975) followed a fixation method which began with washes in cacodylate buffer, followed by incubation of thin sections in a peroxidase localization medium and hydrogen peroxide. 2. This was followed by dehydration in graded ethanols and embedding in S p u r r ' s medium (Spurr, 1969). By using this m e t h o d , Hoff and G a u b a t z (1975) were successful in localizing apo-B in h u m a n intracranial arteries. The most significant single contribution made by electron microscopy to the understanding of the morphologic events in atherosclerosis is the characterization of the spindle cells located in the intima of atherosclerotic vessels of experimental animals as being " m o d i f i e d " smooth muscle cells (Parker, 1960; Parker and O d l a n d , 1966).
This filamentous material from adjacent cells frequently merges to form an uninterrupted basement m e m b r a n e . However, in the course of the atherogenic process, the base ment m e m b r a n e of smooth muscle cells often appears discontinuous and focal in distribution. , 1966). Vracko and Benditt (1970) observed that endothelial cell injury stimulates basal lamina for mation. Apparently, regeneration of the endothelial cells results in a specific cell product associated with basal lamina reduplication, an obser vation first reported by Majno (1965) and in accordance with the observa tion made in this laboratory (Jones, 1976).
Advances in lipid research. Vol. 18, 1981 by Rodolfo Paoletti; David Kritchevsky