By Rodolfo Paoletti, Dr. David Kritchevsky
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Extra info for Advances in lipid research. Volume 6
The bottom 4 ml fraction now contains the V H D L and all other serum proteins. 001 M EDTA at p H 8. 01 volume of the thimerosal stock solution described above unless they are to be studied immediately. 35 Plasma Lipoprotein Analysis An alternative procedure (Radding and Steinberg, 1960) may be used for occasions when dilution of samples must be kept to a minimum. Density adjustments are made with solid KBr according to the following calculation: ndt-ck) (4) x=- l-Vd, where X is the gm solid KBr to be added for adjustment, V4 is the initial volume of solution to be adjusted, df is the final density desired, d, is the initial density, and V is the partial specific volume of KBr (see Fig.
P R E P A R A T I O N O F S E R U M O R P L A S M A F O R ANALYSIS Lipoproteins are moderately unstable substances and become particularly so when, during fractionation procedures, they become separated from their normal environment of serum proteins and small molecules. , 1954). Although the molecular mechanisms of deterioration are not understood, the result is a progressive alteration in chemical, ultracentrifugal, or electrophoretic behavior. A plasma enzyme which transfers fatty acids from phospholipid to cholesterol (Glomset, 1962) and a system which exchanges lipids between H D L and VLDL (Nichols and Smith, 1965) may contribute other changes in lipoprotein composition and properties during storage.
25 in a 1-cm cell. 90 for a considerable period. It is believed that during this period reproducible staining may be obtained. For quantitative work band intensities might be corrected for changes in the absorbance of the dye. 90, regeneration may b e accomplished by heating the dye solution to boiling under a condenser, but this regeneration can be done only once. 891 at 37°C. Plasma Lipoprotein 6. 41 Analysis Care ofElectrophoresis Cell An important part of obtaining reproducible lipoprotein electrophoresis is the routine maintenance of the electrophoresis cell.
Advances in lipid research. Volume 6 by Rodolfo Paoletti, Dr. David Kritchevsky